Recombinant proteins can be produced through the recombinant DNA technology, which involves the insertion of genes (gene splicing) into the genetic material of bacteria or mammalian cells. These organisms are used as “factories” as they can rapidly replicate and thus, produce large volume of proteins within a short period of time.
The recombinant DNA molecule must undergo a number of replication to provide material for analysis and sequencing. Cloning is the production of many identical copies of the same recombinant DNA molecule. Cloning can be done in vitro by polymerase chain reaction (PCR). It can also be done in vivo in unicellular microbes such as E. coli, unicellular eukaryotes like yeast, and in mammalian cells grown in cell culture.
The expression of recombinant proteins require a cloning vector that replicates within the living cell. A vector serves as a “transport vehicle” for the production of proteins translated from specific DNA sequences. Vectors are usually derived from plasmids and viruses, naturally exist from bacterial and mammalian cells, respectively.
Recombinant protein technology can be used to treat diseases involving minimal expressions of specific proteins, for instance, Type I Diabetes. They are also widely used as reagents in laboratory experiments and to generate antibody probes for examining protein synthesis within cells and organisms.
Esco VacciXcell Solution
Esco VacciXcell offers a wide range of bioreactors and fermenters that can facilitate the cultivation of adherent and suspension cells. Esco VacciXcell’s tide motion bioreactors, CelCradle™ and TideCell®, differ in volume production capacity. Esco VacciXcell also offers various sizes of fermenters/stirred tank bioreactor, starting from 5L laboratory scale up to 2000L production scale.
Esco VacciXcell offers solutions for the low-cost production of biosimilars, beginning from process development up to large-scale production, without compromising quality. Thanks to Esco’s biosafety expertise, VacciXcell offers not only process equipment, but as well as turnkey laboratories and facilities.