Applications Vaccixcell

BSL3/4 Facilities

Bioprocessing requires handling of live microorganisms and viruses, which may pose significant and even life-threatening risks to the operator and the environment and therefore, must be properly contained. The appropriate isolation and handling of these biological agents depend on the level of biohazard they pose.

Biosafety Level 1 (BSL-1) agents are those ones that do not cause any known disease to a healthy adult. Biosafety Level 2 (BSL-2) agents are agents associated with human diseases that are transmitted via ingestion, mucous or secretion exposure or percutaneous injuries. Biosafety Level 3 (BSL-3) agents involve exotic and endemic agents, which may possibly cause serious to lethal diseases through intranasal exposure, while Biosafety Level 4 (BSL-4) agents include exotic agents that pose life-threatening diseases through aerosol or unknown transmissions.

Facilities for BSL 3 and BSL 4 agents, therefore, require the most stringent conditions to ensure complete operator and environment protection. These facilities, most especially those handling BSL-4 agents, must be located in a separate building or in a controlled area of a building, isolated from other and they must meet a list of rigorous regulatory standards.

Esco VacciXcell Advantage

Esco VacciXcell offers various Class III cabinets, suited for users’ different operating and budget requirements. These cabinets meet international regulatory standards including, American Standard NSF 49, European Standard EN 12469, Australian Standard AS 2252, and Japanese Standard JIS K 3800. Moreover, Esco VacciXcell’s partner companies, Esco and Tapestle Rx, also specialize in cleanroom technology and in the designing and building of laboratory facilities, respectively. Together with these two companies, Esco VacciXcell can facilitate constructions of BSL-3 and BSL-4 facilities.

Related Products

Airstream® Class III Biosafety Cabinet




Aerobiology is the branch under biology that focuses on the study of bacteria, spores, pollen grains, viruses and other organic particles that is airborne by nature which can be considered as infecting agents. In order to properly deal with these airborne substances and particles, aerobiological studies and experimentation must be performed under specialized environments. We at VacciXcell know and understand the need to protect not only the research products and subjects but more importantly the person handling the research. Being a world leader in innovative isolation technology, our class III biological cabinets offer the following personnel and product protection:

  • Double-filtered high quality ULPA filtration to ensure that no contamination may happen outside of the work zone, ensuring the safety and welfare of every lab personnel
  • Exclusive dual exhaust filters provide >100,000 times better protection than single-stage designs.
  • Integrated pass through interlocking doors provides a method of material transfer without compromising work zone isolation and lab safety

  • Negative pressure environment inside the cabinet provides another level of security in the off-chance that there may be a breach in the isolation system; giving peace of mind to the operator to perform to the best of his abilities without worrying about personal well being

VacciXcell products adhere to international standards such as American Standard NSF 49, European Standard EN 12469, Australian Standard AS 2252, and Japanese Standard JIS K 3800 among others. VacciXcell is also capable of providing customized solutions that will cater to your processing needs.

To learn more about our class III biological safety cabinets:

If you desire an even higher degree of isolation technology, check out VacciXcell's Containment Barrier Isolator and Isoclean Platforms.

VacciXcell's Class III biological safety cabinets is one of the most cost effective solutions available in the market today. Being able to operate in a relatively small footprint, Airstream® Class III biological safety cabinets can fit smoothly into your laboratory specially when space is premium luxury. Airstream® also boast of a user friendly ergonomic design attributed to its angled cabinet front feature allowing an ergonomic working posture for the operator.

VacciXcell Class III biological safety cabinet is the most cost-effective and space optimizing solution for laboratories, whether it is used for capacity expansion, limited intermittent uses or as reserved units. It is capable of handling high risk materials and unknown hazards without compromising operator safety.  VacciXcell Class III biological safety cabinet is a safe, effective and value-for-money acquisition to solve complex laboratory isolation requirements.

Airstream® Class III biological safety cabinet standard features include:

ESCO Airstream®  Class III BSC
  • Double-filtered exhaust air through high-quality ULPA filters (per IEST-RP-CC-001.3) with typical efficiency of >99.999% for 0.1 to 0.3 micron particles, better than HEPA filters.
  • Exclusive dual exhaust filters provide >100,000 times better protection than single-stage designs.
  • Microprocessor-based Esco Sentinel™ Silver control system provides visual / audible alarms for airflow
  • Esco ISOCIDE antimicrobial surface on all painted surfaces minimizes contamination
  • An integrated pass-through with interlocking doors, permits materials transfer without risk of environmental contamination

To learn more about Airstream®, click here.

To learn more about other isolation technology options, check out VacciXcell's Containment Barrier Isolator and Isoclean Platforms.

Facilities that handle unknown substances with potential of being high-risk, such as departments involved in homeland security or health governing departments, must be equipped with proper isolation technology to have a safe and secure containment of the substances. VacciXcell class III biological safety cabinets are well equipped to provide the containment requirements of such facilities. VacciXcell's containment barrier isolator (CBI) Provides Operator Exposure Levels (OEL's) ?1.0 ?g/m3 during controlled operations providing optimum operator safety for substances such as toxic industrial chemicals (TIC), toxic industrial material (TIM) and Non-Traditional Agents. Aside from these agents, there are also highly infectious microbiological agents which needs to be handled properly. Manipulation of substances within the CBI is made possible by the one piece leak tight sleeve-glove. CBI facilitates the complete isolation of substances that is processed inside while providing sterile/aseptic environment at the same time; ensuring that contamination on either side will not be a problem. On top of this, the CBI can be configured and integrated with whatever customized function that your process might require.

As chemicals and biological substances become more and more complex, facilities handling transportation and analysis of these biological and/or chemical substances must also be technologically updated. Let VacciXcell be your partner in innovative isolation technology

Containment Barrier Isolator standard features:

  • Controls exposure/cross-contamination risk to hazardous/aseptic materials for a wide variety of equipment and processes
  • Controls false-positive risk for sterility testing
  • Provides Operator Exposure Levels (OEL's) ?1.0 ?g/m3 during controlled operations
  • Levels of ?0.1 ?g/m3 can be achieved via closed-transfer processes or based on client SOPs
  • Enhances cGMP practices
  • Quiet, energy-efficient ECM fans auto adjust to compensate for filter blockage
  • Standard dimensions available are easily customized to suit process requirements
  • Safe glove change and low contamination filter change
  • FDA-approved static seals
  • Pressure tested class 1 ISO 10648-2 standards
  • System comes with a Semi-automated or Automated Pressure Hold Testing
  • Pass Chamber comes in 2 sizes:
    • Small, non-gloved
    • Large, non-gloved/gloved

To learn more about other isolation technology options, check out VacciXcell's Isoclean Platforms or VacciXcell's Airstream®.

We at VacciXcell understand that every laboratory has its own preference, whether it be in its process or equipment which sometimes causes integration issues when it comes to upgrading or adding additional equipment. VacciXcell provides not only the equipment required for various laboratory and industry applications but also provide the engineering knowledge and expertise to integrate this into an existing system or to build it from the ground up. VacciXcell in partnership with TapestleRx can provide whole clean room solutions that will cater to your specifications. Both the equipment and the cleanroom adheres to the most stringent international standards in existence.

VacciXcell can provide you a customized concept-to-completion engineering solution with world class project management for both equipment and facility. Each biological safety cabinet made by VacciXcell is guaranteed to have complied and passed stringent leak, pressure and airflow testing. Each cabinet is also equipped with the necessary HEPA/ULPA/Carbon filters are required by customer application. VacciXcell also provides the following value added service to its customers:

  • 3D Computed aided drawing (CAD) of initial design
  • Factory acceptance testing (FAT) and Site acceptance testing (SAT)
  • IQ/OQ/PQ as required
  • Complete and comprehensive documentation as required by clients
  • Trained and skilled engineering personnel for installation, maintenance and troubleshooting
  • On-site training of customer personnel

For more information about our isolation technology products visit:

Traditionally, in order to be able to do formulation and filling, whole clean rooms must be prepared. Clean room construction is not only costly but also takes a several months, if not years, to construct. Aside from this, the maintenance and operation cost of having a cleanroom is another financial burden that a manufacturer needs to consider. This has hindered pharmaceutical industries in the past due to the heavy financial commitment required. Nowadays, isolators are being accepted as a cheaper alternative to these cleanrooms. An isolator can easily be commissioned within a few days after on-site delivery and can be used immediately after. Initial cost and maintenance cost of an isolator is also relatively cheaper as compared to completing a whole clean room facility.

VacciXcell class III biological safety cabinets and isolators are able to provide the aseptic environment required for pharmaceutical formulation and filling. Using the latest innovative technologies in filter and airflow control, VacciXcell cabinets and isolators guarantees sterility with absolutely no containment breach under normal use. Furthermore, VacciXcell's innovative technology combines robotics and isolation containment bringing filling line technology to a whole new level. AseptiCell™ is a fully integrated and isolated system for pharmaceutical filling into ready-to-use containers.

Learn more about Asepticell and  VacciXcell barrier isolator.

Extracting, isolation and preparing seed cells for cell culture might provide some aseptic challenge if not done in a proper environment. Seed cells might get contaminated by unwanted foreign objects such as dust or airborne pathogens which would result in undesired products or total failure of the cell culture. On the other hand, cells with pathogenic properties and/or are infected with pathogens which may cause human or environmental harm must be kept isolated. Whichever the case may be, VacciXcell has the capability to supply the isolation containment that you require. Whether it be an aseptic isolator to protect your cells or containment isolator to prevent environmental contamination, VacciXcell has the products for you.

Aside from our standard products, we also cater to customized isolation solutions depending on your requirement. If you want to learn more about our containment barrier visit this page


VacciXcell also enjoys being a leader in cell culture technological innovation. VacciXcell TideCell bioreactor uses emerge-submerge principle of cell culture wherein aeration and media submersion of the cells are done alternatively, giving the cells the best environment to grow and multiply without having to worry about foaming. This technology combined with our patented BioNOCII micro-carriers provides more surface area per volume of micro-carrier, allowing higher cell density in a relatively small amount of space.

To learn more about VacciXcell bioreactors, click here.

Why settle for one if you can get both? Combining our superior and innovative bioreactor system with the aseptic containment guarantee of our barrier systems, we bring you the Cradlepro Iso. Now no cell culture process will be compromised, whether it is seed cell preparation or the actual cell culture, nor will environmental and operator safety be compromised in any way.

Cradlepro Iso

Pre-clinical stage of vaccine development involves animal testing. Usual animal subjects are mice or monkeys wherein the vaccine will be tested whether it could invoke an immune response or not. By doing this, researchers of the effect it might have on human cells. Infecting animals with infectious and potentially harmful pathogens creates the risk of outbreaks in case of containment breach. As such, it is ideal that these experiments be done inside a barrier isolator with negative pressure.

VacciXcell's containment barrier isolator (CBI) not only provides superior operator protection but also protects the welfare of the animal from external factors while being infected. The CBI boasts of operator exposure levels (OEL's) ?1.0 ?g/m3 during controlled operations through physical barrier isolation and negative internal pressure. The CBI also comes with an integrated pass chamber which allows the entry and exit of the subjects without breaking containment.

To learn more about CBI, click here.

Containment or isolation in an agricultural setting focuses on preventing the escape of subjects, whether plants or animals, under study to the environment which might have a negative impact on the environment, whether it be through soil, air or water. These subjects might be carriers of high risk pathogens or pathogenic agents as well as genetic modification which might have unknown consequences.  Although operator safety and public health is always a point of consideration, focus of the containment is still on preventing the subject of study, or any substance thereof, from escaping into the environment.

In another context, containment might also be used in veterinary applications such as industrialized farming to avoid pathogenic outspread in the case of livestock epidemic for example. In this case, groups or herds of animals are put into containment such that 1) the pathogens of infected animals are contained 2) healthy animals will not be infected.

VacciXcell can provide the containment solution for any of these scenario. Our class III biological safety cabinets can be configured for either positive pressure for maximum product protection or negative pressure for maximum operator and environmental protection.

To learn more about our containment products: