2D Culture system to 3D Closed System
In-vitro cell culture has been traditionally done using two dimensional (2D) monolayer culture. Example of 2D static culture devices include petri dishe, t-flask, roller bottle and Hyperstack.
2D culture is appealing to researchers for a variety of reasons. For one, the environment within a 2D static culture device is easy to control. Usually all that is required is putting the device inside a CO2 in order to control the culture environment.
Being monolayer, cell observation can easily be done. Visual inspection is possible since there is no third dimension that might be blocking or hiding other cells within the culture. Further cell observation can be virtually done by using a simple microscope.
Another advantage of 2D culture is that, being the traditional method, a lot of published articles and journals discussing 2D culture model has been written and made available to the public. This factor makes it appealing for new researchers or researchers doing a new project to use 2D culture method due to the wide availability of resources.
With all this being said, 2D culture poses some concerns as well. Firstly, 2D culture methods provides a very limited culture space. The only way to scale up in 2D culture methods is to scale up the size of the device as well. This is a very limiting factor specially for production scale.
Second, 2D culture does not mimic the in-vivo conditions of the body. This unnatural environment would cause cells to be unnatural as well. 2D culture serves its purpose if the goal is to study cell biology as observation is simplified. But for more complex applications such as for drug testing or expressions (gene or protein), the cells would not be a viable subject.
Third, 2D culture is exposed to a lot of contamination risks. 2D culture methods require manual processes such as seeding, routine media change, sampling, and harvesting.
VacciXcell's Closed System TideMotion Bioreactor
The Tidemotion Bioreactor is a 3D culture system that fully solves all of the limitations of a 2D system. Being 3D, scaling up in tidemotion bioreactors will not entail a proportional scale up in the reactor size. It is possible to culture a density of 1 trillion cells in a small room. Culture environment inside the tidemotion bioreactor also mimics in-vivo conditions of cells, thus making their properties and characteristics as similar as possible to natural cells, making the cells suitable for complex applications. Also, being a closed system, tidemotion bioreactors negate the possibility of contamination and makes the work environment that much safer.
To learn more about VacciXcell's Tidemotion Bioreactors: