BioNOC™ Sampling for Process Development
Tide motion systems, through the use of BioNOC™ II macrocarriers, allow individual carrier sampling for PAT/IPQC purposes. Cell counting can be done by sampling individual BioNOC™ II and staining it with crystal violet dye (CVD). This process can be done with a few simple steps:
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Randomly pick 6 carriers using a sterilized forceps and place them on a sterilized petri dish.
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Place two carriers in one vial.
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Vortex each vial for 60 sec.
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Place vials into a 37? incubator for 1 hour. Vortex several times during incubation.
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Dilute samples with PBS to a countable range
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Vortex vials and pipette 10ul diluted CVD solution for counting. Place the plate under a phase contrast microscope and count the nucleus (will be shown in bright purple colors)