Technology Vaccixcell

Tide Motion Technology

VacciXcell's tide motion system is intended for the culture of adherent or anchorage-dependent cells to be used for various applications such as cell banking, cell culture-based vaccine production, biologics production, cell therapy, gene therapy and diagnostics.

Tide motion technology derived its name from the cyclical low and high tide experienced by the bodies of water here on earth. Similarly, tide motion uses this upward and downward liquid flow to provide both oxygen and nutrients to the adherent cells being cultured. The gentle vertical oscillation of the culture medium creates a dynamic interface between air and culture medium on the surface of the cells, providing the cells with an environment that is of low shear stress, high aeration and nutrition levels, zero foaming, and no O2 limitation. This efficient nutrient and oxygen transfer is what allows the tide motion system to produce high density cell yield

To better understand, let us begin with the basics of the technology. There are two main parts of the tide motion system namely the packed bed and the media reservoir. Let us take the lab scale tide motion bioreactor CelCradleTM bottle as an example:

The bellow of the bottle would then be gently compressed and decompressed, creating the "tide motion". This tide motion come in two phases: nutrition and aeration.

Nutrition Phase

 

During nutrition phase, the bellow is compressed thus pushing the media upward and into the packed bed above. This action allows the media to interact with the cells attached to the packed providing them the necessary nutrients.

Aeration Phase

 

During aeration phase, the bellow is decompressed allowing the media to flow back down thus exposing the packed bed to air. This stage allows oxygenation to the cells in a direct manner as compared to merely relying on dissolved oxygen within the media.

This cycle continues until the desired number of cells is achieved. Tide motion allows high density culture of cells without the risk of cell death due to lack of resources.