Technology Vaccixcell

VacciXcell's Macrocarrier (BioNOC II)


Anchorage-dependent cells, as the name implies, require a surface to which they adhere to and grow. These cells are typically grown in t-flasks or roller bottles but the difficulty in scale up of these culture vessels have led to the development of carriers. Carriers can take various forms and sizes; some can be spherical in shape called microcarrier beads, which are used in stirred tank bioreactors, while some can be in the form of sheets or fibers, which are used in packed-bed or tide motion bioreactor. A wide variety of carriers are available in the market today, each differing in their physical and chemical properties. The choice of carrier depend greatly on the type of cells to be cultured, as different cells have varying anchorage requirements.


Acting as the packed bed component of the tide motion system is the BioNOC™ II, which is a 100% PET fibrous culture matrix with stability of up to 5 years.

BioNOC™ II Macrocarrier

Actual photo of BioNOC™ II


These macrocarriers provide cells with a large surface area for attachment and growth and 3D matrix that closely mimics a cell's in vivo environment.

Microscopic view: cell attached to BioNOC™ II


Furthermore, the efficient oxygenation and nutrition exchange in the matrix vessel and protection of cells from shear stress allows cells to proliferate well and produce high yields of bioproducts.

Illustration of how cells are situated within BioNOC™ II


The BioNOC™ II macrocarriers also have very low fibre content as compared to other macrocarrers thereby eliminating otherwise necessary downstream processing steps

Comparison of BioNOC™ II lint content



BioNOC™ II cell culture matrix are carriers for the growth of anchorage-dependent animal, mammalian, and insect cells. BioNOC™ II supports the growth of different cell lines, including CHO, CHO-K1, rCHO-hIgG, rC-127-TPA, HEK293, Hybridoma, Vero, Sf-9, Hi-5, BHK-21, rBHK Factor VIII, HepG2, Hela, Huh 7, RK-13, human foreskin fibroblasts, human muscle skeleton cells, human mesenchymal cells, and human embryonic stem cells.